A continuous culture is at steady state with HRT24 h and growth rate μ₁. HRT is reduced to 12 h, and a new non-washout steady state has rate μ₂. Which relation holds?
In the intended chemostat without cell retention or decay, steady biomass balance requires μ=D=1/HRT. Thus μ₁=1/24 and μ₂=1/12 h⁻¹=2μ₁. Equivalently **μ₁=.5μ₂,B**. The premise that a new steady culture survives excludes washout.
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