A 15 ng/μL DNA solution in a 0.5 mm microcuvette has absorbance 0.24 at 260 nm. After concentration, absorbance is 0.38 at the same wavelength and path length. Find final concentration in ng/μL to two decimals.
Beer–Lambert law makes absorbance proportional to concentration when path length and absorptivity stay fixed. Thus c2/c1=A2/A1=0.38/0.24. Final concentration is 15×0.38/0.24=23.75 ng/μL.
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