GATE 2022 BT – Question 14
Match the media component (Column I) with its role (Column II).
| Column I | Column II |
|---|---|
| P. Sucrose | 1. Anti-foam agent |
| Q. Zinc chloride | 2. Nitrogen source |
| R. Ammonium sulphate | 3. Carbon source |
| S. Silicone oil | 4. Trace element |
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Correct answer: (D) P-3, Q-4, R-2, S-1
Explanation
Match each medium component with the role it plays in a fermentation or culture medium.
- **P. Sucrose:** a sugar used as the **carbon (and energy) source**. P-3.
- **Q. Zinc chloride:** zinc is needed in tiny amounts as a cofactor of enzymes, so it is a **trace element**. Q-4.
- **R. Ammonium sulphate:** supplies $NH_4^+$ as the **nitrogen source**. R-2.
- **S. Silicone oil:** a surface-active liquid added to break up foam, an **anti-foam agent**. S-1.
Match: **P-3, Q-4, R-2, S-1** (option D).