GATE Fundamentals of Biological Engineering: Upstream Processing: Media formulation and optimization, sterilization of air and media – Previous Year Questions
8 GATE previous year questions on Upstream Processing: Media formulation and optimization, sterilization of air and media (Fundamentals of Biological Engineering, Biotechnology) with answers and explanations, from every paper.
- GATE 2021 BT Q32 – A bacterial strain has decimal reduction time 20 min. Specific death rate constant (min⁻¹, two decimal places) is .
- GATE 2022 BT Q14 – Match the media component (Column I) with its role (Column II). Column I Column II --- --- P. Sucrose 1. Anti-foam agent Q. Zinc chloride 2. Nitrogen…
- GATE 2022 BT Q63 – A 100 m³ medium initially contains 108 spores/mL. The accepted final contamination is one spore in the vessel and death constant is 2\ min-1 at 121°C.…
- GATE 2026 BT Q59 – A batch sterilizer is being operated at 121 oC for sterilizing a medium containing microbial cells. Assume that the thermal deactivation of cells is a…
- GATE 2026 BT Q62 – It is desired to cultivate 150 mg (dry weight) microbial cells with an empirical formula of C 5H 7O 2N. If the phosphorus requirement for the cells is…
- GATE 2025 BT Q61 – A hot, freshly-sterilised fermentation medium is cooled in a double-pipe heat-exchanger. The medium enters the inner pipe of the exchanger at 95 oC…
- GATE 2024 BT Q35 – The decimal reduction time of a microbe during sterilization at 120 oC with a first order thermal death rate constant of 1 min-1 will be min (rounded…
- GATE 2024 BT Q54 – Fermentation medium is cooled from 121 oC to 30 oC in a double pipe heat exchanger. If cold water is flowing in the counter-current direction and is…