The GATE Grind

GATE Recombinant DNA Technology and Other Tools in Biotechnology: Analytical tools: Microscopy and spectroscopy – Previous Year Questions

10 GATE previous year questions on Analytical tools: Microscopy and spectroscopy (Recombinant DNA Technology and Other Tools in Biotechnology, Biotechnology) with answers and explanations, from every paper.

  1. GATE 2021 BT Q44 (2 marks, Multiple choice) – Tertiary structure of a protein with α-helices and β-strands can be determined by
  2. GATE 2021 BT Q48 (2 marks, Multiple select) – Which nuclei are NMR active?
  3. GATE 2021 BT Q63 (2 marks, Numerical answer) – DNA at 50 μg/mL has A260=1.0. A 20 μL aliquot from 50 μL purified plasmid is diluted to 1000 μL and gives A260=0.550. Purified plasmid concentration…
  4. GATE 2022 BT Q45 (2 marks, Multiple choice) – Which of the following spectroscopic technique(s) can be used to identify all the functional groups of an antibiotic contaminant in food? P. Infrared…
  5. GATE 2023 BT Q28 (1 mark, Multiple choice) – Which one of the following methods CANNOT be used to determine the secondary structure content of a protein?
  6. GATE 2023 BT Q57 (2 marks, Numerical answer) – A protein has three identical sites at the vertices of an equilateral triangle. One donor has quantum yield D=0.5. Adding one acceptor reduces it to…
  7. GATE 2025 BT Q60 (2 marks, Numerical answer) – The percentage of light that would pass through a sample with an absorbance of 2 would be %. (Round off to the nearest integer)
  8. GATE 2025 BT Q64 (2 marks, Numerical answer) – An NMR spectrometer operating at proton resonance frequency () of 1 GHz will have a magnetic field strength of Tesla (T). The gyromagnetic ratio for…
  9. GATE 2024 BT Q11 (1 mark, Multiple choice) – In adsorption chromatography, the adsorption of uncharged solute molecules onto a silica-based stationary phase is by .
  10. GATE 2024 BT Q41 (2 marks, Multiple choice) – Match the item in Column I with the corresponding technique in Column II. Column I Column II --- --- P. Blue laser 1. Electron microscopy Q. Tungsten…