GATE 2019 BT – Question 42

Recombinant DNA Technology and Other Tools in Biotechnology · Analytical tools: Microscopy and spectroscopy · 2 marks · Multiple choice

Match methods P infrared, Q circular dichroism, R nuclear magnetic resonance with applications 1 functional-group identification, 2 secondary structure, 3 molecular-weight estimation, 4 three-dimensional structure.

P–4, Q–3, R–1
P–2, Q–1, R–3
P–1, Q–2, R–4
P–3, Q–2, R–4

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Show answer and explanation

Correct answer: (C) P–1, Q–2, R–4

Explanation

IR vibrational bands identify functional groups, P–1. Far-UV circular dichroism reports protein secondary-structure content, Q–2. NMR constraints can determine biomolecular three-dimensional structures, R–4. The matching is C.

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