GATE 2019 BT – Question 37

Recombinant DNA Technology and Other Tools in Biotechnology · Molecular tools: Site-directed mutagenesis, CRISPR-Cas, biosensors, interaction tools, genomics and proteomics approaches · 2 marks · Multiple choice

In site-directed mutagenesis, which restriction enzyme digests methylated template DNA?

KpnI
DpnI
XhoI
MluI

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Show answer and explanation

Correct answer: (B) DpnI

Explanation

DpnI recognizes methylated GATC sites and can remove the methylated parental plasmid after amplification of an unmethylated mutant product. Thus B. This selective template digestion is why DpnI appears in the mutagenesis workflow rather than an ordinary sequence-cutting enzyme without this methylation requirement.

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