The GATE Grind

GATE 2026 BT – Question 26

Recombinant DNA Technology and Other Tools in Biotechnology · Molecular tools: Site-directed mutagenesis, CRISPR-Cas, biosensors, interaction tools, genomics and proteomics approaches · 1 mark · Multiple choice

Which component of the CRISPR/Cas9 gene editing system is NOT of natural origin?

  1. CAS9 protein
  2. CRISPR repeats
  3. PAM sequence
  4. sgRNA

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Correct answer: (D) sgRNA

Explanation

Cas9, the CRISPR repeats and the PAM sequence are found in the bacterial immune system. The single guide RNA (sgRNA) is an engineered fusion of the natural crRNA and tracrRNA, so it is the artificial part.