The GATE Grind

GATE 2024 BT – Question 46

Recombinant DNA Technology and Other Tools in Biotechnology · Molecular tools: PCR, DNA and RNA labelling, Sanger and next generation sequencing, Southern and northern blotting, in-situ hybridization, DNA fingerprinting · 2 marks · Multiple select

Which of the following features help(s) in distinguishing alleles using restriction fragment length polymorphism (RFLP)?

  1. Differences in the number of recognition sites for a given restriction enzyme
  2. Differences in the ability of alleles to undergo recombination
  3. Differences in the ability of alleles to undergo segregation
  4. Differences in the number of tandem repeats

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Correct answer: (A) Differences in the number of recognition sites for a given restriction enzyme; (D) Differences in the number of tandem repeats

Explanation

RFLP detects alleles that differ in fragment length, either because a restriction site is gained or lost or because the number of tandem repeats between sites differs.