GATE Recombinant DNA Technology and Other Tools in Biotechnology: Molecular tools: PCR, DNA and RNA labelling, Sanger and next generation sequencing, Southern and northern blotting, in-situ hybridization, DNA fingerprinting – Previous Year Questions

6 GATE previous year questions on Molecular tools: PCR, DNA and RNA labelling, Sanger and next generation sequencing, Southern and northern blotting, in-situ hybridization, DNA fingerprinting (Recombinant DNA Technology and Other Tools in Biotechnology, Biotechnology) with answers and explanations, from every paper.

1. GATE 2022 BT Q26 (1 mark, Multiple choice) – Among individuals in a human population, minor variations exist in nucleotide sequences of chromosomes. These variations can lead to gain or loss of…
2. GATE 2023 BT Q24 (1 mark, Multiple choice) – Which of the following methods are used for detection of DNA and RNA, respectively?
3. GATE 2023 BT Q65 (2 marks, Numerical answer) – A dNTP master mix combines 40 μL of each 20 mM dATP, dCTP, dGTP and dTTP stock. 4 μL of this mix is added to a PCR mix of final volume 50 μL. The…
4. GATE 2024 BT Q23 (1 mark, Multiple choice) – Which one of the following is the basic principle of Sanger's DNA sequencing method?
5. GATE 2024 BT Q40 (2 marks, Multiple choice) – Match the chemical (Column I) with its use (Column II). Column I Column II --- --- P. Diethylpyrocarbonate 1. Chelation of magnesium ion during DNA…
6. GATE 2024 BT Q46 (2 marks, Multiple select) – Which of the following features help(s) in distinguishing alleles using restriction fragment length polymorphism (RFLP)?