The GATE Grind

GATE 2024 BT – Question 40

Recombinant DNA Technology and Other Tools in Biotechnology · Molecular tools: PCR, DNA and RNA labelling, Sanger and next generation sequencing, Southern and northern blotting, in-situ hybridization, DNA fingerprinting · 2 marks · Multiple choice

Match the chemical (Column I) with its use (Column II).

Column IColumn II
P. Diethylpyrocarbonate1. Chelation of magnesium ion during DNA purification
Q. Cesium chloride2. Prevention of RNA degradation in aqueous environment
R. Ethidium bromide3. Separation of DNA by density gradient centrifugation
S. Ethylenediaminetetraacetic acid4. Staining of RNA in agarose gel
  1. P-4; Q-1; R-3; S-2
  2. P-4; Q-3; R-2; S-1
  3. P-2; Q-1; R-4; S-3
  4. P-2; Q-3; R-4; S-1

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Correct answer: (D) P-2; Q-3; R-4; S-1

Explanation

DEPC inactivates RNases (2), CsCl forms the density gradient (3), ethidium bromide stains nucleic acids in the gel (4) and EDTA chelates divalent ions such as magnesium (1).