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GATE 2024 BT – Question 39

Plant, Animal and Microbial Biotechnology · Animals: Culture media and growth conditions, preservation, anchorage dependent and independent culture, growth kinetics, carrier culture · 2 marks · Multiple choice

Match the item (Column I) with its corresponding use (Column II).

Column IColumn II
P. Glutamine1. Detachment of adherent cells
Q. Trypsin2. Selection of transfected mammalian cell lines
R. Hypoxanthine3. Source of carbon and nitrogen in animal cell culture media
S. Neomycin4. A component of medium for selection of hybridoma in monoclonal antibody production
  1. P-3; Q-1; R-4; S-2
  2. P-1; Q-2; R-4; S-3
  3. P-3; Q-1; R-2; S-4
  4. P-2; Q-3; R-1; S-4

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Correct answer: (A) P-3; Q-1; R-4; S-2

Explanation

Glutamine is the carbon and nitrogen source (3), trypsin detaches adherent cells (1), hypoxanthine is in HAT medium for selecting hybridomas (4) and neomycin (G418) selects transfected cells (2).