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GATE 2019 BT – Question 45

Plant, Animal and Microbial Biotechnology · Animals: Culture media and growth conditions, preservation, anchorage dependent and independent culture, growth kinetics, carrier culture · 2 marks · Multiple choice

Which fetal-bovine-serum functions in animal-cell culture are CORRECT? P stimulates growth; Q enhances attachment; R supplies hormones/minerals; S maintains pH at 7.4.

  1. P and Q only
  2. P and S only
  3. P, Q and R only
  4. P, Q, R and S

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Show answer and explanation

Correct answer: (C) P, Q and R only

Explanation

Serum supplies growth-promoting factors, attachment-supporting proteins and nutrients including hormones/minerals, making P,Q,R true. A fixed pH of 7.4 is maintained by the medium’s buffering and gas-control conditions, not guaranteed by serum. Therefore C excludes S.

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