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GATE 2025 BT – Question 20

Plant, Animal and Microbial Biotechnology · Animals: Culture media and growth conditions, preservation, anchorage dependent and independent culture, growth kinetics, carrier culture · 1 mark · Multiple choice

Which one of the following hosts is used in mammalian cell culture for the production of glycosylated recombinant therapeutic proteins?

  1. Pichia pastoris
  2. Sf9 cells
  3. Escherichia coli
  4. Chinese hamster ovary cells

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Correct answer: (D) Chinese hamster ovary cells

Explanation

Chinese hamster ovary (CHO) cells are the standard mammalian host for therapeutic glycoproteins. Pichia is a yeast, Sf9 cells are insect cells and E. coli does not glycosylate proteins.