The GATE Grind

GATE 2020 BT – Question 21

Recombinant DNA Technology and Other Tools in Biotechnology · Recombinant DNA technology: Restriction and modification enzymes, vectors, expression vectors, libraries, gene isolation and cloning, transposons, gene targeting · 1 mark · Multiple choice

Which statement about Agrobacterium Ti plasmid is CORRECT?

  1. Vir genes are located within the T-DNA segment.
  2. Phytohormone biosynthesis genes are located outside the T-DNA segment.
  3. Opine catabolism genes are located within the T-DNA segment.
  4. Opine biosynthesis genes are located within the T-DNA segment.

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Correct answer: (D) Opine biosynthesis genes are located within the T-DNA segment.

Explanation

The transferred T-DNA carries genes that induce plant hormone and opine synthesis. The bacterium retains virulence machinery outside T-DNA to mediate transfer, and its opine-catabolism genes enable it to use the resulting opines. Hence opine biosynthesis genes lie within T-DNA, D; the other statements reverse the usual locations.

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