Lentiviral vectors reverse-transcribe their genome and use integrase to insert the transgene into host chromosomal DNA, enabling stable expression. Thus C fits the requested high-frequency integration. Adenoviral and herpes-based vectors are commonly maintained outside chromosomes; recombinant AAV generally persists predominantly as episomes rather than furnishing this integration mechanism.
More Recombinant DNA technology: Restriction and modification enzymes, vectors, expression vectors, libraries, gene isolation and cloning, transposons, gene targeting questions
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