GATE Recombinant DNA Technology and Other Tools in Biotechnology: Recombinant DNA technology: Restriction and modification enzymes, vectors, expression vectors, libraries, gene isolation and cloning, transposons, gene targeting – Previous Year Questions

6 GATE previous year questions on Recombinant DNA technology: Restriction and modification enzymes, vectors, expression vectors, libraries, gene isolation and cloning, transposons, gene targeting (Recombinant DNA Technology and Other Tools in Biotechnology, Biotechnology) with answers and explanations, from every paper.

1. GATE 2021 BT Q19 (1 mark, Multiple choice) – Which technique is NOT used for inserting a foreign gene into a cell?
2. GATE 2021 BT Q25 (1 mark, Multiple choice) – The enzyme adding phosphate to the free 5′ terminus of DNA is
3. GATE 2021 BT Q64 (2 marks, Numerical answer) – The possible number of SalI restriction sites in 9 kb double-stranded DNA with equiprobable bases (nearest integer) is .
4. GATE 2022 BT Q25 (1 mark, Multiple choice) – The recognition sequences of four Type-II restriction enzymes (RE) are given below. The symbol (↓) indicates the cleavage site. Identify the RE that…
5. GATE 2022 BT Q64 (2 marks, Numerical answer) – A circular plasmid has three different but unique restriction sites for enzymes ‘a’, ‘b’ and ‘c.’ When enzymes ‘a’ and ‘b’ are used together, two…
6. GATE 2023 BT Q45 (2 marks, Multiple select) – Which of the following vector(s) is(are) used to clone a DNA fragment of size 220 kb?