A crude enzyme preparation has protein concentration 10 mg/mL. A 10 μL aliquot gives activity 5 μmol/min under standard conditions. Find specific activity in units/mg.
The aliquot volume is 0.010 mL, so protein mass is 10×0.010 = 0.10 mg. One unit is 1 μmol/min under the stated assay. Thus the aliquot has 5 units and specific activity $5/0.10=50$ units/mg.
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