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GATE Fundamentals of Biological Engineering: Downstream Processing: Filtration, centrifugation, cell disruption, chromatography, extraction, adsorption and drying – Previous Year Questions

8 GATE previous year questions on Downstream Processing: Filtration, centrifugation, cell disruption, chromatography, extraction, adsorption and drying (Fundamentals of Biological Engineering, Biotechnology) with answers and explanations, from every paper.

  1. GATE 2021 BT Q38 (2 marks, Multiple choice) – Match P centrifugation, Q dialysis, R solvent extraction and S ultrafiltration to: 1 density, 2 diffusivity, 3 size, 4 solubility.
  2. GATE 2021 BT Q57 (2 marks, Numerical answer) – A 100 cm sedimentation tank separates spherical sludge of diameter 0.5 mm. Liquid viscosity is 1 cP and density difference 0.1 g/cm³. Assuming…
  3. GATE 2022 BT Q40 (2 marks, Multiple choice) – Match the stationary phase (Column I) with its corresponding chromatography technique (Column II). Column I Column II --- --- P. Protein A 1. Size…
  4. GATE 2023 BT Q49 (2 marks, Numerical answer) – Aqueous two-phase extraction is used to recover α-amylase from a solution. A polypropylene glycol-dextran mixture is added and the solution separates…
  5. GATE 2026 BT Q19 (1 mark, Multiple choice) – Which one of the following proteins would elute third in gel filtration chromatography from a mixture of four proteins given below?
  6. GATE 2025 BT Q23 (1 mark, Multiple select) – Which of the following separation processes is/are based on molecular size?
  7. GATE 2025 BT Q27 (1 mark, Multiple select) – A recombinant protein is secreted extracellularly in soluble form by an E. coli culture. Which of the following downstream processes is/are involved…
  8. GATE 2025 BT Q57 (2 marks, Numerical answer) – A binary mixture of benzene and toluene under vapour-liquid equilibrium at 80 oC follows ideal Raoult's law. At this condition, the saturation…