GATE 2022 BT – Question 40
Match the stationary phase (Column I) with its corresponding chromatography technique (Column II).
| Column I | Column II |
|---|---|
| P. Protein A | 1. Size exclusion chromatography |
| Q. Sephadex | 2. Ion-exchange chromatography |
| R. Phenylsepharose | 3. Affinity chromatography |
| S. Diethylaminoethyl cellulose | 4. Hydrophobic interaction chromatography |
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Correct answer: (B) P-3, Q-1, R-4, S-2
Explanation
Match each stationary phase (column material) with the chromatography it is used in.
- **P. Protein A:** binds the Fc region of antibodies specifically, so it is used for **affinity chromatography**. P-3.
- **Q. Sephadex:** a cross-linked dextran gel with defined pore sizes that separates by size, used for **size exclusion (gel filtration)**. Q-1.
- **R. Phenyl-sepharose:** has hydrophobic phenyl groups, used for **hydrophobic interaction chromatography**. R-4.
- **S. Diethylaminoethyl (DEAE) cellulose:** a positively charged anion exchanger, used for **ion-exchange chromatography**. S-2.
Match: **P-3, Q-1, R-4, S-2** (option B).