The GATE Grind

GATE 2019 BT – Question 39

Recombinant DNA Technology and Other Tools in Biotechnology · Computational tools: Bioinformatics resources, databases, sequence analysis, alignment and phylogeny · 2 marks · Multiple choice

For a BLAST protein-database search, which statements are CORRECT? P larger E-value means higher sequence similarity; Q $E<10^{-10}$ indicates sequence homology; R higher BLAST score means higher sequence similarity; S $E>10^{10}$ indicates sequence homology.

  1. P, Q and R only
  2. Q and R only
  3. P, R and S only
  4. P and S only

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Correct answer: (B) Q and R only

Explanation

A smaller E-value means fewer alignments of that quality are expected by chance, so it gives stronger evidence for homology; a higher score indicates a stronger alignment. Hence Q and R are the intended correct statements, B. E-value significance provides evidence rather than a logical proof of evolutionary homology, and depends on search context; larger E-values do not support the claim in P or S.

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