The GATE Grind

GATE 2025 BT – Question 55

Recombinant DNA Technology and Other Tools in Biotechnology · Analytical tools: Electrophoresis, microarrays, enzymatic assays, immunoassays, immunoblotting, flow cytometry, whole genome and ChIP sequencing · 2 marks · Multiple select

Which of the following methods is/are used for identifying histone modifications?

  1. ChIP-seq
  2. Mass spectrometry
  3. Immunofluorescence
  4. Patch-clamp electrophysiology

Practise this question in The GATE Grind →

Show answer and explanation

Correct answer: (A) ChIP-seq; (B) Mass spectrometry; (C) Immunofluorescence

Explanation

ChIP-seq with modification-specific antibodies maps them on the genome, mass spectrometry identifies them on the histones, and immunofluorescence shows them in cells. Patch-clamp measures ion channel currents.