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GATE Fundamentals of Biological Engineering: Bioreaction Engineering: Batch, fed-batch and continuous processes, microbial and enzyme reactors, optimization and scale up – Previous Year Questions

23 GATE previous year questions on Bioreaction Engineering: Batch, fed-batch and continuous processes, microbial and enzyme reactors, optimization and scale up (Fundamentals of Biological Engineering, Biotechnology) with answers and explanations, from every paper.

  1. GATE 2021 BT Q52 (2 marks, Numerical answer) – A chemostat has D=0.8\ h-1, biomass 8 mol/m³ and specific product formation 0.2 mol product/(mol biomass h). Steady product concentration (mol/m³) is…
  2. GATE 2021 BT Q55 (2 marks, Numerical answer) – Fermentation is scaled from 1 L to 1000 L at constant power/volume. Small-vessel agitation is 300 rpm and linear scale factor is 10. Large-vessel…
  3. GATE 2021 BT Q61 (2 marks, Numerical answer) – Batch E. coli has zero-order Monod growth. At termination dissolved O2 is 10% saturation and k La=80\ h-1. Saturation is 0.007 kg/m³, maximum growth…
  4. GATE 2022 BT Q58 (2 marks, Numerical answer) – A batch ends with 50 g biomass. Fed-batch feeding is 1 L/h of 50 g/L substrate, with maximum yield 0.4 g/g. Under quasi-steady conditions, maximum…
  5. GATE 2023 BT Q48 (2 marks, Numerical answer) – E. coli is cultivated in a chemostat at dilution rate 0.2\ h-1. Biomass yield due to oxygen consumption is 0.2\ g/g and steady biomass concentration…
  6. GATE 2023 BT Q53 (2 marks, Numerical answer) – The schematic shows a chemostat with cell recycle. F 0,F r are feed and recycle flow rates, and X 1,X 0,X are reactor, recycle and product cell…
  7. GATE 2026 BT Q44 (2 marks, Multiple select) – A microbial culture is being grown in a bioreactor with continuous aeration. The dissolved oxygen probe provided with the system shows a reading of…
  8. GATE 2026 BT Q45 (2 marks, Multiple select) – For a Newtonian fluid, a plot of shear stress (on Y axis) against shear rate (on X axis) will result in a straight line .
  9. GATE 2026 BT Q46 (2 marks, Multiple select) – In an aerobic fermentation with air sparging, which of the following options can be used to increase the volumetric mass transfer coefficient for…
  10. GATE 2026 BT Q47 (2 marks, Multiple select) – For a liquid flowing in a circular cross section pipe, the transition from laminar to turbulent flow will NOT depend on which of the following…
  11. GATE 2026 BT Q56 (2 marks, Numerical answer) – A CSTR with a volume of 100 m3 is operated in cell recycle mode. At a volumetric flow rate of 10 m3 day-1 and effluent biomass of 20 mg L-1, the…
  12. GATE 2026 BT Q61 (2 marks, Numerical answer) – A microbe that follows Monod growth kinetics on a limiting substrate (maximum specific growth rate of 0.5 h-1 and Monod constant of 0.1 mg L-1) is…
  13. GATE 2026 BT Q65 (2 marks, Numerical answer) – A packed bed bioreactor with length 1 m and inside diameter 10 cm has liquid flowing at an interstitial velocity of 1 cm s-1. Given a volumetric flow…
  14. GATE 2025 BT Q25 (1 mark, Multiple select) – Which of the following fluids exhibit(s) non-Newtonian behaviour at 25 oC?
  15. GATE 2025 BT Q29 (1 mark, Numerical answer) – For a mechanically reversible isobaric process taking place in a closed system involving 5 moles of an ideal gas, the temperature increases from an…
  16. GATE 2025 BT Q44 (2 marks, Multiple choice) – For a specific bioreactor configuration, the power requirement for a Rushton-turbine impeller agitating an unaerated Newtonian fluid in the turbulent…
  17. GATE 2025 BT Q62 (2 marks, Numerical answer) – A 2 L bioreactor is being operated as a chemostat, at a flow rate of 0.8 L/h and sterile feed of 10 g/L substrate. The bacterial growth follows Monod…
  18. GATE 2024 BT Q13 (1 mark, Multiple choice) – Which one of the following statements is correct in the context of thermodynamics?
  19. GATE 2024 BT Q14 (1 mark, Multiple choice) – Which one of the following statements is correct about Reynolds Number (N Re) in a stirred tank bioreactor?
  20. GATE 2024 BT Q53 (2 marks, Multiple select) – Under complete cell washout condition in a chemostat with sterile feed, which of the following statements is/are correct?
  21. GATE 2024 BT Q56 (2 marks, Numerical answer) – Ethanol is produced in a 10,000 L stirred bioreactor using an impeller of diameter 1 m. The density and viscosity of fermentation broth are 1000 kg…
  22. GATE 2024 BT Q59 (2 marks, Numerical answer) – A fermentor is filled with medium at a rate of 1 L min-1. A leak develops at the bottom of the fermentor when the medium in the fermentor reaches 200…
  23. GATE 2024 BT Q61 (2 marks, Numerical answer) – Consider scale-up of fungal fermentation from a 20 L model-type to 20,000 L prototype stirred tank reactor. The model-type and prototype have the same…