The GATE Grind

GATE 2020 BT – Question 43

Recombinant DNA Technology and Other Tools in Biotechnology · Computational tools: Bioinformatics resources, databases, sequence analysis, alignment and phylogeny · 2 marks · Multiple choice

Carl Woese used the gene sequence of which molecule for phylogenetic taxonomy of prokaryotes?

  1. Ribosomal RNA of the large ribosomal subunit
  2. Ribosomal RNA of the small ribosomal subunit
  3. Ribosomal protein of the large ribosomal subunit
  4. Ribosomal protein of the small ribosomal subunit

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Correct answer: (B) Ribosomal RNA of the small ribosomal subunit

Explanation

Small-subunit ribosomal RNA, specifically 16S rRNA in prokaryotes, combines conserved regions with informative sequence variation and is broadly comparable across organisms. Woese’s rRNA comparisons established major evolutionary relationships, including the distinction of archaea. Select B; the marker is RNA of the small subunit, not a ribosomal protein.

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