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GATE 2025 BT – Question 48

Plant, Animal and Microbial Biotechnology · Plants: Hairy root culture, artificial seeds, somaclonal variation, protoplast fusion, transgenic plants, marker genes, plastid transformation · 2 marks · Multiple choice

Correctly match the herbicide with its mode of development of resistance in plants.

HerbicideMode of development of resistance
P. Imidazolinones1. Transformation of bacterial nitrilase gene
Q. Bromoxynil2. Transformation of resistant version of acetolactate synthetase
R. Glufosinate3. Transformation of tfdA gene from Alcaligenes, which encodes a dioxygenase
4. Transformation of bar gene from Streptomyces hygroscopicus which encodes phosphinothricin acetyltransferase
  1. P-2; Q-1; R-4
  2. P-2; Q-1; R-3
  3. P-1; Q-2; R-3
  4. P-4; Q-1; R-3

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Correct answer: (A) P-2; Q-1; R-4

Explanation

Imidazolinones inhibit acetolactate synthase, and resistance comes from a resistant version of the enzyme (2). Bromoxynil is detoxified by a bacterial nitrilase (1). Glufosinate (phosphinothricin) is inactivated by the acetyltransferase encoded by the bar gene (4). The tfdA gene gives resistance to 2,4-D.