E. coli is grown for several generations in $^{15}N$ medium, transferred to $^{14}N$ medium and allowed four generations before DNA isolation. Find the proportion of intermediate-density DNA, rounded to 2 decimals.
After the first semiconservative replication, all duplexes contain one old heavy strand and one new light strand. Each later generation doubles total DNA while the number of heavy-containing duplexes remains fixed. After four generations the hybrid fraction is $1/2^{4-1}=1/8=0.125$, approximately 0.13 to two decimal places.
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